Recombinant Mouse R-Spondin 1 Protein, CF (3474-RS) (2024)

Catalog # 3474-RS | R&D Systems, Inc. a Bio-Techne Brand

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Citations (65)

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Citations & Reviews
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Scientific Data
Background
Product Documents
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Key Product Details

Source

E. coli

Accession #

Q9Z132

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse R-Spondin 1 protein
Ser21-Gly209, with an N-terminal Met

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

21 kDa

Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The typical ED₅₀ is50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.

Scientific Data Images for Recombinant Mouse R-Spondin 1 Protein, CF

Recombinant Mouse R-Spondin 1 Protein Bioactivity

Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS) induces activation of beta-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 50-200 ng/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Recombinant Mouse R-Spondin 1 Protein SDS-PAGE

1 μg/lane of Recombinant Mouse R-Spondin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.

Formulation, Preparation and Storage

3474-RS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).

Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.

References

Chen, J-Z.et al.(2002) Mol. Biol. Rep.29:287.
Kim, K.-A.et al.(2006) Cell Cycle5:23.
Nam, J.-S.et al.(2007) Gene Expr.Patterns7:306.
Binnerts, M.E.et al.(2007) Proc. Natl. Acad.Sci. USA104:14700.
Nam, J.-S.et al.(2006) J. Biol. Chem.281:13247.
Wei, Q.et al.(2007) J. Biol. Chem.282:15903.
Kamata, T.et al.(2004) Biochim. Biophys.Acta1676:51.
Parma, P.et al.(2006) Nat. Genet.38:1304.
Kim, K.-A.et al.(2005) Science309:1256.
Zhao, J.et al.(2007) Gastroenterology132:1331.
Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
Kazanskaya, O.et al.(2004) Dev. Cell7:525.
Tomaselli, S.et al.(2008) Hum. Mutat.29:220.

Long Name

Roof Plate-specific Spondin 1

Alternate Names

Cristin 3, CRISTIN3, HRspo1, RSPO, RSPO1, RSpondin 1

Entrez Gene IDs

284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)

Gene Symbol

RSPO1

UniProt

Q9Z132

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Product Documents for Recombinant Mouse R-Spondin 1 Protein, CF

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COA

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Product Specific Notices for Recombinant Mouse R-Spondin 1 Protein, CF

For research use only

Citations
Reviews

Citations for Recombinant Mouse R-Spondin 1 Protein, CF (65)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse R-Spondin 1 Protein, CF include:

Species: Mouse
Sample Types: Whole Tissue
Applications: Organoid Culture

Jiang et al. (2023-10-26), Microbial-dependent recruitment of immature myeloid cells promotes intestinal regeneration Cellular and molecular gastroenterology and hepatology
PMID: 37898454
Species: Mouse
Sample Types: Organoids

Hung et al. (2023-10-26), Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model BMC genomics
PMID: 37884859
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

X Huang et al. (2023-04-13), Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization Nature Communications, 2023-014(1):2104.
PMID: 37055389
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

TT Lemmetyine et al. (2023-04-03), Fibroblast-derived EGF ligand Neuregulin-1 induces fetal-like reprogramming of the intestinal epithelium without supporting tumorigenic growth Disease Models & Mechanisms, 2023-00(0).
PMID: 36912192
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

E Domènech-M et al. (2023-03-13), Tellu: an object detector algorithm for automatic classification of intestinal organoids Disease Models & Mechanisms, 2023-00(0).
PMID: 36804687
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

JW Villanueva et al. (2022-12-01), Comprehensive microRNA analysis across genome-edited colorectal cancer organoid models reveals miR-24 as a candidate regulator of cell survival BMC Genomics, 2022-123(1):792.
PMID: 36457077
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Viladomiu et al. (2022-11-15), Agr2-associated ER stress promotes adherent-invasive E.ï¿½coli dysbiosis and triggers CD103+ dendritic cell IL-23-dependent ileocolitis Cell Reports, 2022-141(7):111637.
PMID: 36384110
Species: Mouse
Sample Types: Organoids

K Kumar Mani et al. (2022-10-31), Intestinal Epithelial Stem Cell Transplants as a Novel Therapy for Cerebrovascular Stroke Brain, Behavior, and Immunity, 2022-10(0).
PMID: 36328163
Species: Mouse
Sample Types: Organoids
Applications: Bioassay

S Watanabe et al. (2022-02-02), Transplantation of intestinal organoids into a mouse model of colitis Nature Protocols, 2022-00(0).
PMID: 35110738
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Han et al. (2022-01-11), Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1 Cell Reports, 2022-038(2):110240.
PMID: 35021086
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

JR Knight et al. (2021-12-13), Rpl24Bst mutation suppresses colorectal cancer by promoting eEF2 phosphorylation via eEF2K Elife, 2021-110(0).
PMID: 34895463
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Tarquis-Me et al. (2021-11-20), Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas International Journal of Molecular Sciences, 2021-122(22).
PMID: 34830411
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

SR Taylor et al. (2021-08-18), Dietary fructose improves intestinal cell survival and nutrient absorption Nature, 2021-00(0).
PMID: 34408323
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

JDG Leach et al. (2021-06-08), Oncogenic BRAF, unrestrained by TGF&beta-receptor signalling, drives right-sided colonic tumorigenesis Nature Communications, 2021-012(1):3464.
PMID: 34103493
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Bioassay

A Böttcher et al. (2021-01-04), Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates Nature Cell Biology, 2021-023(1):23-31.
PMID: 33398177
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

AJ Bilotta et al. (2020-11-22), Propionate Enhances Cell Speed and Persistence to Promote Intestinal Epithelial Turnover and Repair Cell Mol Gastroenterol Hepatol, 2020-10(0).
PMID: 33238220
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

S Claudinot et al. (2020-11-06), Tp63-expressing adult epithelial stem cells cross lineages boundaries revealing latent hairy skin competence Nat Commun, 2020-111(1):5645.
PMID: 33159086
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Li et al. (2020-10-13), Volumetric Compression Induces Intracellular Crowding to Control Intestinal Organoid Growth via Wnt/&beta-Catenin Signaling Cell Stem Cell, 2020-10(0).
PMID: 33053374
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

RT Zwiggelaar et al. (2020-09-11), LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium Science Advances, 2020-06(37).
PMID: 32917713
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Zhang et al. (2020-08-20), Elevating EGFR-MAPK program by a nonconventional Cdc42 enhances intestinal epithelial survival and regeneration JCI Insight, 2020-05(16).
PMID: 32686657
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

T Han et al. (2020-06-16), Lineage reversion drives WNT independence in intestinal cancer Cancer Discov, 2020-00(0).
PMID: 32546576
Species: Mouse
Sample Types: Whole Cells
Applications: IHC Control

M Zhang et al. (2020-03-11), Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs PLoS ONE, 2020-015(3):e0229445.
PMID: 32160239
Species: Mouse
Sample Types: Whole Tissue
Applications: Organoid Culture

T Xing et al. (2019-12-23), Tight junction protein claudin-7 is essential for intestinal epithelial stem cell self-renewal and differentiation Cell Mol Gastroenterol Hepatol, 2019-10(0).
PMID: 31874254
Species: Mouse
Sample Types: Whole Tissue
Applications: Tissue Culture
See Also
R-Spondin 1 Products | Bio-Techne

AP Singh et al. (2019-11-19), Enteroendocrine progenitor cell enriched miR-7 regulates intestinal epithelial proliferation in an Xiap-dependent manner Cell Mol Gastroenterol Hepatol, 2019-10(0).
PMID: 31756561
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Yung et al. (2019-10-11), Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals Nat Commun, 2019-110(1):4647.
PMID: 31604927
Species: Mouse
Sample Types: Tissue Explants
Applications: Tissue Culture

X Wang et al. (2019-10-07), Wnt Signaling Protects against Pacl*taxel-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea In Vitro Biomed Res Int, 2019-12019(0):7878906.
PMID: 31687397
Species: Mouse
Sample Types: Tissue hom*ogenates
Applications: Tissue Culture

EM Schatoff et al. (2019-07-23), Distinct CRC-associated APC mutations dictate response to Tankyrase inhibition Cancer Discov, 2019-00(0).
PMID: 31337618
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

KK Dickinson et al. (2019-04-12), Molecular determinants of WNT9b responsiveness in nephron progenitor cells PLoS ONE, 2019-014(4):e0215139.
PMID: 30978219
Species: Mouse
Applications: Bioassay

Z Meng et al. (2019-02-07), The atypical antipsychotic quetiapine induces hyperlipidemia by activating intestinal PXR signaling JCI Insight, 2019-04(3).
PMID: 30728326
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

F Chen et al. (2018-08-31), Neutrophils Promote Amphiregulin Production in Intestinal Epithelial Cells through TGF-? and Contribute to Intestinal Homeostasis J. Immunol., 2018-00(0).
PMID: 30171165
Species: Human
Sample Types: Organoids
Applications: Bioassay

M Boyd et al. (2018-04-25), Characterization of the enhancer and promoter landscape of inflammatory bowel disease from human colon biopsies Nat Commun, 2018-09(1):1661.
PMID: 29695774
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

MJ Kim et al. (2018-03-12), PAF-Myc-Controlled Cell Stemness Is Required for Intestinal Regeneration and Tumorigenesis Dev. Cell, 2018-044(5):582-596.e4.
PMID: 29533773
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

NR Smith et al. (2018-03-10), Monoclonal Antibodies Reveal Dynamic Plasticity Between Lgr5-ï¿½and Bmi1-Expressing Intestinal Cell Populations Cell Mol Gastroenterol Hepatol, 2018-06(1):79-96.
PMID: 29928673
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay

T Takahashi et al. (2018-03-05), The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice Int J Mol Sci, 2018-019(3).
PMID: 29510587
Species: Mouse
Sample Types: Whole Cell
Applications: Bioassay

Y Saito et al. (2018-02-19), Development of a functional thyroid model based on an organoid culture system Biochem. Biophys. Res. Commun., 2018-00(0).
PMID: 29470983
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay

S Yui et al. (2017-12-14), YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration Cell Stem Cell, 2017-122(1):35-49.e7.
PMID: 29249464
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

MA Scavuzzo et al. (2017-09-07), Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells Sci Rep, 2017-07(1):10810.
PMID: 28883507
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

EC Moorefield et al. (2017-08-29), Aging effects on intestinal homeostasis associated with expansion and dysfunction of intestinal epithelial stem cells Aging (Albany NY), 2017-09(8):1898-1915.
PMID: 28854151
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

PJ Attayek et al. (2016-04-21), In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment PLoS ONE, 2016-011(4):e0153795.
PMID: 27100890
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Morrison G et al. (2015-12-16), Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification. EMBO J, 2015-135(3):356-68.
PMID: 26675138
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Huels D et al. (2015-08-03), E-cadherin can limit the transforming properties of activating beta-catenin mutations. EMBO J, 2015-034(18):2321-33.
PMID: 26240067
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Calderon-Gierszal E et al. (2015-07-29), Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure. PLoS ONE, 2015-010(7):e0133238.
PMID: 26222054
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Barrett C et al. (2015-06-08), Selenoprotein P influences colitis-induced tumorigenesis by mediating stemness and oxidative damage. J Clin Invest, 2015-0125(7):2646-60.
PMID: 26053663
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Caruso M et al. (2015-04-24), R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis. PLoS ONE, 2015-010(4):e0124213.
PMID: 25910078
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Moon C et al. (2015-03-11), Compartmentalized accumulation of cAMP near complexes of multidrug resistance protein 4 (MRP4) and cystic fibrosis transmembrane conductance regulator (CFTR) contributes to drug-induced diarrhea. J Biol Chem, 2015-0290(18):11246-57.
PMID: 25762723
Species: Mouse
Sample Types: Tissue hom*ogenates
Applications: Bioassay

Dow et al. (2015-02-18), Inducible in vivo genome editing with CRISPR-Cas9. Nat Biotechnol, 2015-033(4):390-4.
PMID: 25690852
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Lopez-Arribillaga E et al. (2014-12-05), Bmi1 regulates murine intestinal stem cell proliferation and self-renewal downstream of Notch. Development, 2014-1142(1):41-50.
PMID: 25480918
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

McElroy S et al. (2014-09-09), The ErbB4 ligand neuregulin-4 protects against experimental necrotizing enterocolitis. Am J Pathol, 2014-0184(10):2768-78.
PMID: 25216938
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

f*ckuda M et al. (2014-08-15), Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon. Genes Dev, 2014-028(16):1752-7.
PMID: 25128495
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Capaldo C et al. (2014-07-16), Proinflammatory cytokine-induced tight junction remodeling through dynamic self-assembly of claudins. Mol Biol Cell, 2014-025(18):2710-9.
PMID: 25031428
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Jurk D et al. (2014-06-24), Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nat Commun, 2014-02(0):4172.
PMID: 24960204
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Mah et al. (2014-06-10), Impact of diet-induced obesity on intestinal stem cells: hyperproliferation but impaired intrinsic function that requires insulin/IGF1. Endocrinology, 2014-0155(9):3302-14.
PMID: 24914941
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Skoczek D et al. (2014-06-06), Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium. J Immunol, 2014-0193(1):439-51.
PMID: 24907348
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Wiener Z et al. (2014-05-13), Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta. Proc Natl Acad Sci U S A, 2014-0111(21):E2229-36.
PMID: 24825889
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Shi F et al. (2014-05-07), beta-Catenin is required for hair-cell differentiation in the cochlea. J Neurosci, 2014-034(19):6470-9.
PMID: 24806673
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Takemura N et al. (2014-03-18), Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome. Nat Commun, 2014-05(0):3492.
PMID: 24637670
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Bohorquez D et al. (2014-02-26), An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy. PLoS ONE, 2014-09(2):e89881.
PMID: 24587096
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Matsumoto et al. (2014-02-20), A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J, 2014-033(7):702-18.
PMID: 24562386
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Jin et al. (2014-01-04), In vitro multilineage differentiation and self-renewal of single pancreatic colony-forming cells from adult C57BL/6 mice. Stem Cells Dev, 2014-023(8):899-909.
PMID: 24261600
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Li et al. (2013-11-26), LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats. Endocrinology, 2013-1155(2):429-40.
PMID: 24280058
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Huynh D et al. (2013-02-22), CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response. PLoS ONE, 2013-08(2):e56951.
PMID: 23451116
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Jin L et al. (2013-02-19), Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel. Proc Natl Acad Sci U S A, 2013-0110(10):3907-12.
PMID: 23431132
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Jarde T et al. (2012-04-02), In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-ODeltaN89beta-catenin system. Oncogene, 2012-032(7):883-93.
PMID: 22469981
Species: Mouse
Sample Types: Whole Tissue
Applications: Cell Culture

Wong et al. (2012-03-04), Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling. Nat Cell Biol, 2012-014(4):401-8.
PMID: 22388892
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tomaselli S et al. (2011-01-28), Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling. PLoS ONE, 2011-06(1):e16366.
PMID: 21297984

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Customer Reviews for Recombinant Mouse R-Spondin 1 Protein, CF (5)

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5 customer ratings

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4 stars

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Name: Rebecca Cubitt

Application: Cell Proliferation

Verified Customer | Posted 11/30/2018
Recombinant Mouse R-Spondin 1 Protein, CF

Name: Christine Quach

Application: Cell Culture

Verified Customer | Posted 09/08/2017
Recombinant Mouse R-Spondin 1 Protein, CF

Name: Ben McGlaughon

Application: Apoptosis assay

Verified Customer | Posted 09/08/2017
Name: Michael Dougherty

Verified Customer | Posted 04/21/2017
Recombinant Mouse R-Spondin 1 Protein, CF

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 12/15/2016

Used in culture media to differentiate stem cells towards a pancreatic endocrine progenitor cell fate.

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FAQs for Recombinant Mouse R-Spondin 1 Protein, CF

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Q: What are the differences between Recombinant Mouse R-Spondin 1 Protein, CF (Catalog # 3474-RS) and the new version, Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein (Catalog # 7150-RS)?

A: Catalog # 3474-RS is an E. coli cell derived R-Spondin 1 protein with aaSer21-Gly209. The bioactivity is measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a. Additionally, the purity specification for this protein is >90%.
Catalog # 7150-RS is a Chinese Hamster Ovary (CHO) cell derived R-Spondin 1 protein with aaSer21-Gln265. The bioactivity is measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt‑3a. Additionally, the purity specification for this protein is >95%.

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Recombinant Mouse R-Spondin 1 Protein, CF (3474-RS) (2024)

Catalog # 3474-RS | R&D Systems, Inc. a Bio-Techne Brand

Technical Data

Citations & Reviews

FAQs

Calculators & Tools

Key Product Details

Source

Accession #

Conjugate

Applications

Product Specifications

Source

Purity

Endotoxin Level

N-terminal Sequence Analysis

Predicted Molecular Mass

Activity

Scientific Data Images for Recombinant Mouse R-Spondin 1 Protein, CF

Recombinant Mouse R-Spondin 1 Protein Bioactivity

Recombinant Mouse R-Spondin 1 Protein SDS-PAGE

Formulation, Preparation and Storage

3474-RS

Background: R-Spondin 1

References

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional R-Spondin 1 Products

Product Documents for Recombinant Mouse R-Spondin 1 Protein, CF

Product Datasheets

COA

SDS

Product Specific Notices for Recombinant Mouse R-Spondin 1 Protein, CF

Citations for Recombinant Mouse R-Spondin 1 Protein, CF (65)

Customer Reviews for Recombinant Mouse R-Spondin 1 Protein, CF (5)

Recombinant Mouse R-Spondin 1 Protein, CF

Recombinant Mouse R-Spondin 1 Protein, CF

Recombinant Mouse R-Spondin 1 Protein, CF

FAQs for Recombinant Mouse R-Spondin 1 Protein, CF

References