Recombinant Mouse R-Spondin 1 Protein, CF (2024)

Catalog #: 3474-RS
64 Citations 5 Reviews Datasheet / COA / SDS

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Citations (64)

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Reviews (5)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse R-Spondin 1 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED₅₀ is50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.

Source

E. coli-derived mouse R-Spondin 1 protein
Ser21-Gly209, with an N-terminal Met

Accession #

Q9Z132

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

21 kDa

Product Datasheets

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3474-RS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3474-RS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS) induces activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 50-200 ng/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse R-Spondin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.

Reconstitution Calculator

Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).

Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.

References

Chen, J-Z.et al.(2002) Mol. Biol. Rep.29:287.
Kim, K.-A.et al.(2006) Cell Cycle5:23.
Nam, J.-S.et al.(2007) Gene Expr.Patterns7:306.
Binnerts, M.E.et al.(2007) Proc. Natl. Acad.Sci. USA104:14700.
Nam, J.-S.et al.(2006) J. Biol. Chem.281:13247.
Wei, Q.et al.(2007) J. Biol. Chem.282:15903.
Kamata, T.et al.(2004) Biochim. Biophys.Acta1676:51.
Parma, P.et al.(2006) Nat. Genet.38:1304.
Kim, K.-A.et al.(2005) Science309:1256.
Zhao, J.et al.(2007) Gastroenterology132:1331.
Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
Kazanskaya, O.et al.(2004) Dev. Cell7:525.
Tomaselli, S.et al.(2008) Hum. Mutat.29:220.

Long Name

Roof Plate-specific Spondin 1

Entrez Gene IDs

284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)

Alternate Names

Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin hom*olog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1

Related Research Areas

Bone Growth and Remodeling
Cardiovascular System Development
Digestive System Development
Embryonic and Induced Pluripotent Stem Cell Growth Factors
Excretory System Development
Gastrulation and Germ Layer Formation
Growth Factors
Hematopoietic Stem Cell Growth Factors
Immune System Development
Mesenchymal Stem Cell Growth Factors
Morphogens, Embryonic Patterning and Axis Formation
Muscular System Development
Neural Stem Cell Growth Factors
Respiratory System Development
Skeletal System Development
Tissue Regeneration
Wnt Family
Wnt Signaling Modulators

Citations for Recombinant Mouse R-Spondin 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

64 Citations: Showing 1 - 10
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Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model
Authors: Hung, YH;Capeling, M;Villanueva, JW;Kanke, M;Shanahan, MT;Huang, S;Cubitt, R;Rinaldi, VD;Schimenti, JC;Spence, JR;Sethupathy, P;
BMC genomics
Species: Mouse
Sample Types: Organoids
Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization
Authors: X Huang, T Nero, R Weeraseker, KH Matej, A Hinbest, Z Jiang, RF Lee, L Wu, C Chak, J Nijjer, I Gibaldi, H Yang, N Gamble, WL Ng, SA Malaker, K Sumigray, R Olson, J Yan
Nature Communications, 2023-04-13;14(1):2104.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Fibroblast-derived EGF ligand Neuregulin-1 induces fetal-like reprogramming of the intestinal epithelium without supporting tumorigenic growth
Authors: TT Lemmetyine, EW Viitala, L Wartiovaar, T Kaprio, J Hagström, C Haglund, P Katajisto, TC Wang, E Domènech-M, S Ollila
Disease Models & Mechanisms, 2023-04-03;0(0):.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay
Tellu: an object detector algorithm for automatic classification of intestinal organoids
Authors: E Domènech-M, A Brandt, TT Lemmetyine, L Wartiovaar, TP Mäkelä, S Ollila
Disease Models & Mechanisms, 2023-03-13;0(0):.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay
Comprehensive microRNA analysis across genome-edited colorectal cancer organoid models reveals miR-24 as a candidate regulator of cell survival
Authors: JW Villanueva, L Kwong, T Han, SA Martinez, MT Shanahan, M Kanke, LE Dow, CG Danko, P Sethupathy
BMC Genomics, 2022-12-01;23(1):792.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay
Agr2-associated ER stress promotes adherent-invasive E.ï¿½coli dysbiosis and triggers CD103+ dendritic cell IL-23-dependent ileocolitis
Authors: M Viladomiu, M Khounlotha, B Dogan, SF Lima, A Elsaadi, E Cardakli, JG Castellano, C Ng, J Herzog, AA Schoenborn, M Ellermann, B Liu, S Zhang, AS Gulati, RB Sartor, KW Simpson, SM Lipkin, RS Longman
Cell Reports, 2022-11-15;41(7):111637.
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay
Intestinal Epithelial Stem Cell Transplants as a Novel Therapy for Cerebrovascular Stroke
Authors: K Kumar Mani, Y El-Hakim, TE Branyan, N Samiya, S Pandey, MT Grimaldo, A Habbal, A Wertz, F Sohrabji
Brain, Behavior, and Immunity, 2022-10-31;0(0):.
Species: Mouse
Sample Types: Organoids
Transplantation of intestinal organoids into a mouse model of colitis
Authors: S Watanabe, S Kobayashi, N Ogasawara, R Okamoto, T Nakamura, M Watanabe, KB Jensen, S Yui
Nature Protocols, 2022-02-02;0(0):.
Species: Mouse
Sample Types: Organoids
Applications: Bioassay
Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1
Authors: Y Han, A Villarreal, G Gutierrez, Q Nguyen, P Sun, T Wu, B Sui, G Berx, T Brabletz, K Kessenbroc, YA Zeng, K Watanabe, X Dai
Cell Reports, 2022-01-11;38(2):110240.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Rpl24Bst mutation suppresses colorectal cancer by promoting eEF2 phosphorylation via eEF2K
Authors: JR Knight, N Vlahov, DM Gay, RA Ridgway, WJ Faller, C Proud, GR Mallucci, T von der Ha, CM Smales, AE Willis, OJ Sansom
Elife, 2021-12-13;10(0):.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
Authors: M Tarquis-Me, K Scheibner, I González-G, A Bastidas-P, M Sterr, J Jaki, S Schirge, C García-Các, H Lickert, M Bakhti
International Journal of Molecular Sciences, 2021-11-20;22(22):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Dietary fructose improves intestinal cell survival and nutrient absorption
Authors: SR Taylor, S Ramsamooj, RJ Liang, A Katti, R Pozovskiy, N Vasan, SK Hwang, N Nahiyaan, NJ Francoeur, EM Schatoff, JL Johnson, MA Shah, AJ Dannenberg, RP Sebra, LE Dow, LC Cantley, KY Rhee, MD Goncalves
Nature, 2021-08-18;0(0):.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

Oncogenic BRAF, unrestrained by TGF&beta-receptor signalling, drives right-sided colonic tumorigenesis
Authors: JDG Leach, N Vlahov, P Tsantoulis, RA Ridgway, DJ Flanagan, K Gilroy, N Sphyris, EG Vázquez, DF Vincent, WJ Faller, MC Hodder, A Raven, S Fey, AK Najumudeen, D Strathdee, C Nixon, M Hughes, W Clark, R Shaw, S:CORT con, SR van Hooff, DJ Huels, JP Medema, ST Barry, MC Frame, A Unciti-Bro, SJ Leedham, GJ Inman, R Jackstadt, BJ Thompson, AD Campbell, S Tejpar, OJ Sansom
Nature Communications, 2021-06-08;12(1):3464.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates
Authors: A Böttcher, M Büttner, S Tritschler, M Sterr, A Aliluev, L Oppenlände, I Burtscher, S Sass, M Irmler, J Beckers, C Ziegenhain, W Enard, AC Schamberge, FM Verhamme, O Eickelberg, FJ Theis, H Lickert
Nature Cell Biology, 2021-01-04;23(1):23-31.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Propionate Enhances Cell Speed and Persistence to Promote Intestinal Epithelial Turnover and Repair
Authors: AJ Bilotta, C Ma, W Yang, Y Yu, Y Yu, X Zhao, Z Zhou, S Yao, SM Dann, Y Cong
Cell Mol Gastroenterol Hepatol, 2020-11-22;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

Tp63-expressing adult epithelial stem cells cross lineages boundaries revealing latent hairy skin competence
Authors: S Claudinot, JI Sakabe, H Oshima, C Gonneau, T Mitsiadis, D Littman, P Bonfanti, G Martens, M Nicolas, A Rochat, Y Barrandon
Nat Commun, 2020-11-06;11(1):5645.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

Volumetric Compression Induces Intracellular Crowding to Control Intestinal Organoid Growth via Wnt/&beta-Catenin Signaling
Authors: Y Li, M Chen, J Hu, R Sheng, Q Lin, X He, M Guo
Cell Stem Cell, 2020-10-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium
Authors: RT Zwiggelaar, HT Lindholm, M Fosslie, M Terndrup P, Y Ohta, A Díez-Sánch, M Martín-Alo, J Ostrop, M Matano, N Parmar, E Kvaløy, RR Spanjers, K Nazmi, M Rye, F Drabløs, C Arrowsmith, J Arne Dahl, KB Jensen, T Sato, MJ Oudhoff
Science Advances, 2020-09-11;6(37):.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

Elevating EGFR-MAPK program by a nonconventional Cdc42 enhances intestinal epithelial survival and regeneration
Authors: X Zhang, S Bandyopadh, LP Araujo, K Tong, J Flores, D Laubitz, Y Zhao, G Yap, J Wang, Q Zou, R Ferraris, L Zhang, W Hu, EM Bonder, PR Kiela, R Coffey, MP Verzi, II Ivanov, N Gao
JCI Insight, 2020-08-20;5(16):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lineage reversion drives WNT independence in intestinal cancer
Authors: T Han, S Goswami, Y Hu, F Tang, MP Zafra, C Murphy, Z Cao, JT Poirier, E Khurana, O Elemento, JF Hechtman, K Ganesh, R Yaeger, LE Dow
Cancer Discov, 2020-06-16;0(0):.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs
Authors: M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie
PLoS ONE, 2020-03-11;15(3):e0229445.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC Control

Tight junction protein claudin-7 is essential for intestinal epithelial stem cell self-renewal and differentiation
Authors: T Xing, LJ Benderman, S Sabu, J Parker, J Yang, Q Lu, L Ding, YH Chen
Cell Mol Gastroenterol Hepatol, 2019-12-23;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: Organoid Culture

Enteroendocrine progenitor cell enriched miR-7 regulates intestinal epithelial proliferation in an Xiap-dependent manner
Authors: AP Singh, YH Hung, MT Shanahan, M Kanke, A Bonfini, MK Dame, M Biraud, BCE Peck, OO Oyesola, JM Freund, RL Cubitt, EG Curry, LM Gonzalez, GA Bewick, ED Tait-Wojno, NA Kurpios, S Ding, JR Spence, CM Dekaney, N Buchon, P Sethupathy
Cell Mol Gastroenterol Hepatol, 2019-11-19;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: Tissue Culture

Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals
Authors: T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim
Nat Commun, 2019-10-11;10(1):4647.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Wnt Signaling Protects against Pacl*taxel-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea In Vitro
Authors: X Wang, Y Han, M Wang, C Bo, Z Zhang, L Xu, W Liu, H Wang
Biomed Res Int, 2019-10-07;2019(0):7878906.
Species: Mouse
Sample Types: Tissue Explants
Applications: Tissue Culture

Distinct CRC-associated APC mutations dictate response to Tankyrase inhibition
Authors: EM Schatoff, S Goswami, MP Zafra, M Foronda, M Shusterman, BI Leach, A Katti, BJ Diaz, LE Dow
Cancer Discov, 2019-07-23;0(0):.
Species: Mouse
Sample Types: Tissue hom*ogenates
Applications: Tissue Culture

Molecular determinants of WNT9b responsiveness in nephron progenitor cells
Authors: KK Dickinson, LC Hammond, CM Karner, ND Hastie, TJ Carroll, P Goodyer
PLoS ONE, 2019-04-12;14(4):e0215139.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

The atypical antipsychotic quetiapine induces hyperlipidemia by activating intestinal PXR signaling
Authors: Z Meng, T Gwag, Y Sui, SH Park, X Zhou, C Zhou
JCI Insight, 2019-02-07;4(3):.
Species: Mouse
Sample Types:
Applications: Bioassay

Neutrophils Promote Amphiregulin Production in Intestinal Epithelial Cells through TGF-? and Contribute to Intestinal Homeostasis
Authors: F Chen, W Yang, X Huang, AT Cao, AJ Bilotta, Y Xiao, M Sun, L Chen, C Ma, X Liu, CG Liu, S Yao, SM Dann, Z Liu, Y Cong
J. Immunol., 2018-08-31;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Characterization of the enhancer and promoter landscape of inflammatory bowel disease from human colon biopsies
Authors: M Boyd, M Thodberg, M Vitezic, J Bornholdt, K Vitting-Se, Y Chen, M Coskun, Y Li, BZS Lo, P Klausen, P Jan Schwei, AG Pedersen, N Rapin, K Skovgaard, K Dahlgaard, R Andersson, TB Terkelsen, B Lilje, JT Troelsen, AM Petersen, KB Jensen, I Gögenur, P Thielsen, JB Seidelin, OH Nielsen, JT Bjerrum, A Sandelin
Nat Commun, 2018-04-25;9(1):1661.
Species: Human
Sample Types: Organoids
Applications: Bioassay

PAF-Myc-Controlled Cell Stemness Is Required for Intestinal Regeneration and Tumorigenesis
Authors: MJ Kim, B Xia, HN Suh, SH Lee, S Jun, EM Lien, J Zhang, K Chen, JI Park
Dev. Cell, 2018-03-12;44(5):582-596.e4.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

Monoclonal Antibodies Reveal Dynamic Plasticity Between Lgr5-ï¿½and Bmi1-Expressing Intestinal Cell Populations
Authors: NR Smith, JR Swain, PS Davies, AC Gallagher, MS Parappilly, CZ Beach, PR Streeter, IA Williamson, ST Magness, MH Wong
Cell Mol Gastroenterol Hepatol, 2018-03-10;6(1):79-96.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice
Authors: T Takahashi, A Shiraishi, J Murata
Int J Mol Sci, 2018-03-05;19(3):.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay

Development of a functional thyroid model based on an organoid culture system
Authors: Y Saito, N Onishi, H Takami, R Seishima, H Inoue, Y Hirata, K Kameyama, K Tsuchihash, E Sugihara, S Uchino, K Ito, H Kawakubo, H Takeuchi, Y Kitagawa, H Saya, O Nagano
Biochem. Biophys. Res. Commun., 2018-02-19;0(0):.
Species: Mouse
Sample Types: Whole Cell
Applications: Bioassay

YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
Authors: S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen
Cell Stem Cell, 2017-12-14;22(1):35-49.e7.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay

Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells
Authors: MA Scavuzzo, D Yang, M Borowiak
Sci Rep, 2017-09-07;7(1):10810.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Aging effects on intestinal homeostasis associated with expansion and dysfunction of intestinal epithelial stem cells
Authors: EC Moorefield, SF Andres, RE Blue, L Van Landeg, AT Mah, MA Santoro, S Ding
Aging (Albany NY), 2017-08-29;9(8):1898-1915.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment
Authors: PJ Attayek, AA Ahmad, Y Wang, I Williamson, CE Sims, ST Magness, NL Allbritton
PLoS ONE, 2016-04-21;11(4):e0153795.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification.
Authors: Morrison G, Scognamiglio R, Trumpp A, Smith A
EMBO J, 2015-12-16;35(3):356-68.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

E-cadherin can limit the transforming properties of activating beta-catenin mutations.
Authors: Huels D, Ridgway R, Radulescu S, Leushacke M, Campbell A, Biswas S, Leedham S, Serra S, Chetty R, Moreaux G, Parry L, Matthews J, Song F, Hedley A, Kalna G, Ceteci F, Reed K, Meniel V, Maguire A, Doyle B, Soderberg O, Barker N, Watson A, Larue L, Clarke A, Sansom O
EMBO J, 2015-08-03;34(18):2321-33.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure.
Authors: Calderon-Gierszal E, Prins G
PLoS ONE, 2015-07-29;10(7):e0133238.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Selenoprotein P influences colitis-induced tumorigenesis by mediating stemness and oxidative damage.
Authors: Barrett C, Reddy V, Short S, Motley A, Lintel M, Bradley A, Freeman T, Vallance J, Ning W, Parang B, Poindexter S, Fingleton B, Chen X, Washington M, Wilson K, Shroyer N, Hill K, Burk R, Williams C
J Clin Invest, 2015-06-08;125(7):2646-60.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
PLoS ONE, 2015-04-24;10(4):e0124213.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Compartmentalized accumulation of cAMP near complexes of multidrug resistance protein 4 (MRP4) and cystic fibrosis transmembrane conductance regulator (CFTR) contributes to drug-induced diarrhea.
Authors: Moon C, Zhang W, Ren A, Arora K, Sinha C, Yarlagadda S, Woodrooffe K, Schuetz J, Valasani K, de Jonge H, Shanmukhappa S, Shata M, Buddington R, Parthasarathi K, Naren A
J Biol Chem, 2015-03-11;290(18):11246-57.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Inducible in vivo genome editing with CRISPR-Cas9.
Authors: Dow, Lukas E, Fisher, Jonathan, O'Rourke, Kevin P, Muley, Ashlesha, Kastenhuber, Edward R, Livsh*ts, Geulah, Tschaharganeh, Darjus F, Socci, Nicholas, Lowe, Scott W
Nat Biotechnol, 2015-02-18;33(4):390-4.
Species: Mouse
Sample Types: Tissue hom*ogenates
Applications: Bioassay

Bmi1 regulates murine intestinal stem cell proliferation and self-renewal downstream of Notch.
Authors: Lopez-Arribillaga E, Rodilla V, Pellegrinet L, Guiu J, Iglesias M, Roman A, Gutarra S, Gonzalez S, Munoz-Canoves P, Fernandez-Salguero P, Radtke F, Bigas A, Espinosa L
Development, 2014-12-05;142(1):41-50.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

The ErbB4 ligand neuregulin-4 protects against experimental necrotizing enterocolitis.
Authors: McElroy S, Castle S, Bernard J, Almohazey D, Hunter C, Bell B, Al Alam D, Wang L, Ford H, Frey M
Am J Pathol, 2014-09-09;184(10):2768-78.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.
Authors: f*ckuda M, Mizutani T, Mochizuki W, Matsumoto T, Nozaki K, Sakamaki Y, Ichinose S, Okada Y, Tanaka T, Watanabe M, Nakamura T
Genes Dev, 2014-08-15;28(16):1752-7.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Proinflammatory cytokine-induced tight junction remodeling through dynamic self-assembly of claudins.
Authors: Capaldo C, Farkas A, Hilgarth R, Krug S, Wolf M, Benedik J, Fromm M, Koval M, Parkos C, Nusrat A
Mol Biol Cell, 2014-07-16;25(18):2710-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.
Authors: Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender S, Fullard N, Nelson G, Mann J, van de Sluis B, Mann D, von Zglinicki T
Nat Commun, 2014-06-24;2(0):4172.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Impact of diet-induced obesity on intestinal stem cells: hyperproliferation but impaired intrinsic function that requires insulin/IGF1.
Authors: Mah, Amanda T, Van Landeghem, Lauriann, Gavin, Hannah E, Magness, Scott T, Lund, P Kay
Endocrinology, 2014-06-10;155(9):3302-14.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.
Authors: Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A
J Immunol, 2014-06-06;193(1):439-51.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
Proc Natl Acad Sci U S A, 2014-05-13;111(21):E2229-36.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

beta-Catenin is required for hair-cell differentiation in the cochlea.
Authors: Shi F, Hu L, Jacques B, Mulvaney J, Dabdoub A, Edge A
J Neurosci, 2014-05-07;34(19):6470-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome.
Authors: Takemura N, Kawasaki T, Kunisawa J, Sato S, Lamichhane A, Kobiyama K, Aoshi T, Ito J, Mizuguchi K, Karuppuchamy T, Matsunaga K, Miyatake S, Mori N, Tsujimura T, Satoh T, Kumagai Y, Kawai T, Standley D, Ishii K, Kiyono H, Akira S, Uematsu S
Nat Commun, 2014-03-18;5(0):3492.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy.
Authors: Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R
PLoS ONE, 2014-02-26;9(2):e89881.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.
Authors: Matsumoto, Shinji, Fujii, Shinsuke, Sato, Akira, Ibuka, Souji, Kagawa, Yoshinor, Ishii, Masaru, Kikuchi, Akira
EMBO J, 2014-02-20;33(7):702-18.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

In vitro multilineage differentiation and self-renewal of single pancreatic colony-forming cells from adult C57BL/6 mice.
Authors: Jin, Liang, Feng, Tao, Zerda, Ricardo, Chen, Ching-Ch, Riggs, Arthur D, Ku, Hsun Ter
Stem Cells Dev, 2014-01-04;23(8):899-909.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats.
Authors: Li, Ji-Yao, Chai, Biaoxin, Zhang, Weizhen, Fritze, Danielle, Zhang, Chao, Mulholland, Michael
Endocrinology, 2013-11-26;155(2):429-40.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response.
Authors: Huynh D, Akcora D, Malaterre J, Chan C, Dai X, Bertoncello I, Stanley E, Ramsay R
PLoS ONE, 2013-02-22;8(2):e56951.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel.
Authors: Jin L, Feng T, Shih H, Zerda R, Luo A, Hsu J, Mahdavi A, Sander M, Tirrell D, Riggs A, Ku H
Proc Natl Acad Sci U S A, 2013-02-19;110(10):3907-12.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-ODeltaN89beta-catenin system.
Authors: Jarde T, Evans R, McQuillan K, Parry L, Feng G, Alvares B, Clarke A, Dale T
Oncogene, 2012-04-02;32(7):883-93.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling.
Authors: Wong, Vivian W, Stange, Daniel E, Page, Mahalia, Buczacki, Simon, Wabik, Agnieszk, Itami, Satoshi, van de Wetering, Marc, Poulsom, Richard, Wright, Nicholas, Trotter, Matthew, Watt, Fiona M, Winton, Doug J, Clevers, Hans, Jensen, Kim B
Nat Cell Biol, 2012-03-04;14(4):401-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: Cell Culture

Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
PLoS ONE, 2011-01-28;6(1):e16366.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

FAQs

What are the differences between Recombinant Mouse R-Spondin 1 Protein, CF (Catalog # 3474-RS) and the new version, Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein (Catalog # 7150-RS)?
- Catalog # 3474-RS is an E. coli cell derived R-Spondin 1 protein with aaSer21-Gly209. The bioactivity is measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a. Additionally, the purity specification for this protein is >90%.
  Catalog # 7150-RS is a Chinese Hamster Ovary (CHO) cell derived R-Spondin 1 protein with aaSer21-Gln265. The bioactivity is measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt‑3a. Additionally, the purity specification for this protein is >95%.

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