Recombinant Mouse R-Spondin 1 Protein, CF Summary
| Details of Functionality | Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a. |
| Source | E. coli-derived mouse R-Spondin 1 protein |
| Accession # | |
| N-terminal Sequence | Met |
| Protein/Peptide Type | Recombinant Proteins |
| Gene | Rspo1 |
| Purity | >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW | 21 kDa. |
| Publications | Read Publications using 3474-RS in the following applications:
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Packaging, Storage & Formulations
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Purity | >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Reconstitution Instructions | Reconstitute at250 μg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse R-Spondin 1 Protein, CF
- Cristin 3
- CRISTIN3
- FLJ40906Roof plate-specific spondin-1
- HRspo1
- roof plate-specific spondin
- RSPO
- RSPO1
- RSpondin 1
- R-Spondin 1
- R-spondin hom*olog (Xenopus laevis)
- RSPONDIN
- R-spondin1
- R-spondin-1
Background
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).
Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.
- Chen, J-Z.et al.(2002) Mol. Biol. Rep.29:287.
- Kim, K.-A.et al.(2006) Cell Cycle5:23.
- Nam, J.-S.et al.(2007) Gene Expr.Patterns7:306.
- Binnerts, M.E.et al.(2007) Proc. Natl. Acad.Sci. USA104:14700.
- Nam, J.-S.et al.(2006) J. Biol. Chem.281:13247.
- Wei, Q.et al.(2007) J. Biol. Chem.282:15903.
- Kamata, T.et al.(2004) Biochim. Biophys.Acta1676:51.
- Parma, P.et al.(2006) Nat. Genet.38:1304.
- Kim, K.-A.et al.(2005) Science309:1256.
- Zhao, J.et al.(2007) Gastroenterology132:1331.
- Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
- Kazanskaya, O.et al.(2004) Dev. Cell7:525.
- Tomaselli, S.et al.(2008) Hum. Mutat.29:220.
Publications for R-Spondin 1 (3474-RS)(64)
We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.
We have publications tested in 6 applications: Bioassay, Cell Culture, IHC Control, In Vivo, Organoid Culture, Tissue Culture.
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